ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?

All wells would show uniform overdevelopment due to unbound and excess anti-human Ig enzyme conjugate.

Correct!
Since the enzyme which acts on the substrate is in excess, it would turn all the wells a uniform color whether they were truly positive or not.

The Biology Project
University of Arizona
Monday, March 31, 1997
denicew@u.arizona.edu