Western Blot Activity

Question 2

In strip 1, the combining sites of primary antibodies are bound to:

Substrate

INCORRECT.
Substrate is the chromogen which gets dephosphorylated by alkaline phosphatase resulting in a color change and precipitation onto the region of the membrane where the second antibody bound to the primary antibody.

The Biology Project
University of Arizona
Wednesday, Jan 15, 1998
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