Western Blot Activity

Question 2

In strip 1, the combining sites of primary antibodies are bound to:

Second Antibody

INCORRECT.
Second antibody or conjugate in this case is an anti-human Ig coupled to alkaline phosphatase. This antibody binds to the primary antibody. The conjugate binds to the primary antibody after the primary has bound to the antigen.

The Biology Project
University of Arizona
Wednesday, Jan 15, 1998
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