Molecular Genetics of Prokaryotes Problem Set
Problem 10: Phenotype of cells diploid for lac I gene
Correct!
An E. coli strain that is lac I is conjugated with E. coli cells carrying an F' plasmid with the PI lac I DNA sequence on the episome. How would the expression of the lac Z be regulated in the resulting cells that are diploid and heterozygous for the lac I gene (lac I on episome and lac I on the bacterial chromosome)?
A.
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normal regulation of lactose metabolism
The lac I on the F' episome is dominant to the lac I on the bacterial chromosome.
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B.
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constitutive expression of lac Z .
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C.
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inability to synthesize the lac Z gene product, ß-galactosidase.
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D.
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lac Z gene is inducible, but unable to be repressed by high glucose.
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E.
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no synthesis of the lac I gene product, the lac repressor.
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Friday, October 23, 1998
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