Testing for simple sugars: Benedict's Reagent
Left to right: Benedict's reagent (BnR), potato extract + BnR, onion extract + BnR, 5% glucose + BnR.
RESULTS: Aqua-blue to green = negative.
Yellow to orange = positive. Note: this test cannot detect the simple sugar sucrose. |
Testing for starch: Lugol's Iodine
Left to right: Lugol's iodine (LI), starch solution, starch solution + LI.
RESULTS: Yellow-orange = negative.
Purple-black = positive. |
Testing for proteins: Biuret's Reagent
Left to right: Biuret's reagent (BrR), water + BrR, egg albumin solution, egg albumin solution+ BrR.
RESULTS: Denim-blue = negative. Lavender = positive.
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Terminology review: Controls
Water plus Biuret's reagent is a negative control for the protein test. It demonstrates a negative test result (no protein present).
Egg albumin plus Biuret's reagent is a positive control for the protein test. It demonstrates a positive test result (protein is present).
How would you set up similar controls for the other chemical tests?
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